Western blotting analysis using PGRMC1 antibody (Cat#67027, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using PGRMC1 antibody (Cat#67027, 1:1,000). PGRMC1 expression in wild-type (WT) and PGRMC1 shRNA knockdown (KD) A431 cells of total cell lysates. GAPDH serves as a loading control.
Western blotting analysis using PGRMC1 antibody (Cat#67027, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Immunocytochemical staining of A431 cells with PGRMC1 antibody (Cat#67027, 1:50). Top panel: wild type; Bottom panel: shRNA knockdown. Nuclei were stained blue with DAPI. Scale bar: 20 μm. Note: When PGRMC1 was knocked down, A431cell numb›er was greatly reduced.
Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen
A synthesized peptide derived from human PGRMC1
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000 Immunocytochemistry (ICC): 1:50
Note
This product is for research use only.
Data
Western blotting analysis using PGRMC1 antibody (Cat#67027, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using PGRMC1 antibody (Cat#67027, 1:1,000). PGRMC1 expression in wild-type (WT) and PGRMC1 shRNA knockdown (KD) A431 cells of total cell lysates. GAPDH serves as a loading control.
Western blotting analysis using PGRMC1 antibody (Cat#67027, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Immunocytochemical staining of A431 cells with PGRMC1 antibody (Cat#67027, 1:50). Top panel: wild type; Bottom panel: shRNA knockdown. Nuclei were stained blue with DAPI. Scale bar: 20 μm. Note: When PGRMC1 was knocked down, A431cell numb›er was greatly reduced.
Western blotting analysis using PGRMC1 antibody (Cat#67027, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using PGRMC1 antibody (Cat#67027, 1:1,000). PGRMC1 expression in wild-type (WT) and PGRMC1 shRNA knockdown (KD) A431 cells of total cell lysates. GAPDH serves as a loading control.
Western blotting analysis using PGRMC1 antibody (Cat#67027, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Immunocytochemical staining of A431 cells with PGRMC1 antibody (Cat#67027, 1:50). Top panel: wild type; Bottom panel: shRNA knockdown. Nuclei were stained blue with DAPI. Scale bar: 20 μm. Note: When PGRMC1 was knocked down, A431cell numb›er was greatly reduced.
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