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Home>Product>Primary Antibody

KD-Validated NFKB1 Rabbit pAb

#62082

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Knockdown Validated
Western blotting analysis using NFKB1 antibody (Cat#62082). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NFKB1 antibody (Cat#62082, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using NFKB1 antibody (Cat#62082). NFKB1 expression in wild-type (WT) and NFKB1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NFKB1 antibody (Cat#62082, 1:2,500) and HRP-conjugated goat anti-rabbit  secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using NFKB1 antibody (Cat#62082). NFKB1 expression in wild-type (WT) and NFKB1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NFKB1 antibody (Cat#62082, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NFKB1
  • Catalog：
    62082
  • Application：
    WB
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated NFKB1 Rabbit pAb
Aliases NFKB1; Nuclear Factor Kappa B Subunit 1; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear Factor NF-Kappa-B P105 Subuni; NF-KappaB; NFKB-P50; NFkappaBll; NF-KB1; KBF1; DNA-Binding Factor KBF1; EBP-1; P105; P50; Nuclear Factor Kappa-B DNA Binding Subunit; Nuclear Factor NF-Kappa-B P50 Subunit; NF-Kappabeta; NF-Kappa-B1; NFKB-P105; CVID12; NF-KB
Background

Gene Name: NFKB1

NCBI Gene Entry: 4790

UniProt Entry: P19838

Application Information

Molecular Weight: Predicted, 105 kDa; observed, 105 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human NFKB1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Data
  • Western blotting analysis using NFKB1 antibody (Cat#62082). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NFKB1 antibody (Cat#62082, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using NFKB1 antibody (Cat#62082). NFKB1 expression in wild-type (WT) and NFKB1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NFKB1 antibody (Cat#62082, 1:2,500) and HRP-conjugated goat anti-rabbit  secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using NFKB1 antibody (Cat#62082). NFKB1 expression in wild-type (WT) and NFKB1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NFKB1 antibody (Cat#62082, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NFKB1
  • Catalog：
    62082
  • Application：
    WB
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using NFKB1 antibody (Cat#62082). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NFKB1 antibody (Cat#62082, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using NFKB1 antibody (Cat#62082). NFKB1 expression in wild-type (WT) and NFKB1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NFKB1 antibody (Cat#62082, 1:2,500) and HRP-conjugated goat anti-rabbit  secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using NFKB1 antibody (Cat#62082). NFKB1 expression in wild-type (WT) and NFKB1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NFKB1 antibody (Cat#62082, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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