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KD-Validated BCL2 Rabbit pAb

#62121

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Knockdown Validated
Western blotting analysis using BCL2 antibody (Cat#62121). BCL2 expression in wild type (WT) and BCL2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BCL2 antibody (Cat#62121, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    BCL2
  • Catalog:
    62121
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated BCL2 Rabbit pAb
Aliases BCL2; BCL2 Apoptosis Regulator; PPP1R50; Bcl-2; Protein Phosphatase 1, Regulatory Subunit 50; Apoptosis Regulator Bcl-2; B-Cell CLL/Lymphoma 2; BCL2, Apoptosis Regulator
Background

Gene Name: BCL2

NCBI Gene Entry: 596

UniProt Entry: P10415

Application Information

Molecular Weight: Predicted, 26 kDa; observed, 26 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human BCL2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using BCL2 antibody (Cat#62121). BCL2 expression in wild type (WT) and BCL2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BCL2 antibody (Cat#62121, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using BCL2 antibody (Cat#62121). BCL2 expression in wild type (WT) and BCL2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BCL2 antibody (Cat#62121, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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