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Home>Product>Primary Antibody

KD-Validated PRAME Recombinant Rabbit mAb

#62185

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using PRAME antibody (Cat#62185). PRAME expression in wild type (WT) and PRAME shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PRAME antibody (Cat#62185, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PRAME
  • Catalog：
    62185
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated PRAME Recombinant Rabbit mAb
Aliases PRAME; PRAME Nuclear Receptor Transcriptional Regulator; CT130; MAPE; Melanoma Antigen Preferentially Expressed In Tumors; Preferentially Expressed Antigen In Melanoma; Preferentially Expressed Antigen Of Melanoma; Cancer/Testis Antigen 130; Opa-Interacting Protein 4; OIP-4; OIP4; Opa-Interacting Protein OIP4
Background

Gene Name: PRAME

NCBI Gene Entry: 23532

UniProt Entry: P78395

Application Information

Molecular Weight: Predicted, 58 kDa; observed, 49 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB1720

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen Recombinant protein of human PRAME
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using PRAME antibody (Cat#62185). PRAME expression in wild type (WT) and PRAME shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PRAME antibody (Cat#62185, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PRAME
  • Catalog：
    62185
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using PRAME antibody (Cat#62185). PRAME expression in wild type (WT) and PRAME shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PRAME antibody (Cat#62185, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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