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Home>Product>Primary Antibody

IFIT3 Mouse mAb

#6257

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Western blotting analysis using IFIT3 antibody (Cat#6257). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IFIT3 antibody (Cat#6257, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HACAT cells with IFIT3 antibody (Cat#6257, 1:1,000). Nuclei were stained blue with DAPI; IFIT3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of IFIT3 expression in HaCaT cells using IFIT3 antibody (Cat#6257,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, IFIT3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    IFIT3
  • Catalog：
    6257
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name IFIT3 Mouse mAb
Aliases IFIT3; Interferon Induced Protein With Tetratricopeptide Repeats 3; CIG-49; ISG60; RIG-G; IFI60; Interferon-Induced Protein With Tetratricopeptide Repeats 4; Interferon-Induced Protein With Tetratricopeptide Repeats 3; GARG-49; IFIT4; IRG2; Retinoic Acid-Induced Gene G Protein; Interferon-Induced 60 KDa Protein; IFI-60K; IFIT-3; IFIT-4; ISG-60; CIG49; P60; Cig41
Background

Gene Name: IFIT3

NCBI Gene Entry: 3437

UniProt Entry: O14879

Application Information

Molecular Weight: Predicted, 56 kDa; observed, 60 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB8215

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human IFIT3
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using IFIT3 antibody (Cat#6257). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IFIT3 antibody (Cat#6257, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HACAT cells with IFIT3 antibody (Cat#6257, 1:1,000). Nuclei were stained blue with DAPI; IFIT3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of IFIT3 expression in HaCaT cells using IFIT3 antibody (Cat#6257,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, IFIT3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    IFIT3
  • Catalog：
    6257
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using IFIT3 antibody (Cat#6257). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IFIT3 antibody (Cat#6257, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HACAT cells with IFIT3 antibody (Cat#6257, 1:1,000). Nuclei were stained blue with DAPI; IFIT3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of IFIT3 expression in HaCaT cells using IFIT3 antibody (Cat#6257,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, IFIT3.
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