Western blotting analysis using Phosphoglycerate mutase 1 antibody (Cat#62609). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phosphoglycerate mutase 1 antibody (Cat#62609, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Phosphogiycerate mutase 1 antibody (Cat#62609). Phosphogiycerate mutase 1 expression in wild type (WT) and Phosphogiycerate mutase 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Phosphogiycerate mutase 1 antibody (Cat#62609, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Phosphoglycerate mutase 1 expression in C2C12 cells using Phosphoglycerate mutase 1 antibody (Cat#62609, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phosphoglycerate mutase 1.
