Western blotting analysis using solute carrier family 25 member 12 antibody (Cat#62662). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with solute carrier family 25 member 12 antibody (Cat#62662, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using solute carrier family 25 member 12 antibody (Cat#62662). Solute carrier family 25 member 12 expression in wild type (WT) and solute carrier family 25 member 12 (SLC25A12) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with solute carrier family 25 member 12 antibody (Cat#62662, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Solute carrier family 25 member 12 antibody (Cat#62662, 1:1,000). Nuclei were stained blue with DAPI; Solute carrier family 25 member 12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Solute carrier family 25 member 12 expression in C2C12 cells using Solute carrier family 25 member 12 antibody (Cat#62662, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Solute carrier family 25 member 12.
