Western blotting analysis using phospho-STAT3 (Tyr705) antibody (Cat#63234). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-STAT3 (Tyr705) antibody (Cat#63234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using phospho-STAT3 (Tyr705) antibody (Cat#63234). Phospho-STAT3 (Tyr705) expression in wild type (WT) and STAT3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-STAT3 (Tyr705) antibody (Cat#63234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
KD-Validated Phospho-STAT3 (Tyr705) Recombinant Rabbit mAb
Aliases
STAT3; Signal Transducer And Activator Of Transcription 3; APRF; Acute-Phase Response Factor; Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor); DNA-Binding Protein APRF; ADMIO1; ADMIO; HIES
A synthesized peptide derived from human Phospho-STAT3 (Tyr705)
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000
Note
This product is for research use only.
Data
Western blotting analysis using phospho-STAT3 (Tyr705) antibody (Cat#63234). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-STAT3 (Tyr705) antibody (Cat#63234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using phospho-STAT3 (Tyr705) antibody (Cat#63234). Phospho-STAT3 (Tyr705) expression in wild type (WT) and STAT3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-STAT3 (Tyr705) antibody (Cat#63234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using phospho-STAT3 (Tyr705) antibody (Cat#63234). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-STAT3 (Tyr705) antibody (Cat#63234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using phospho-STAT3 (Tyr705) antibody (Cat#63234). Phospho-STAT3 (Tyr705) expression in wild type (WT) and STAT3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-STAT3 (Tyr705) antibody (Cat#63234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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