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Home>Product>Primary Antibody

KD-Validated CRYZL1 Mouse mAb

#63805

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shRNA Knockdown Validated
Western blotting analysis using crystallin zeta like 1 antibody (Cat#63805). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with crystallin zeta like 1 antibody (Cat#63805, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Western blotting analysis using crystallin zeta like 1 antibody (Cat#63805). Crystallin zeta like 1 expression in wild-type (WT) and crystallin zeta like 1 (CRYZL1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with crystallin zeta like 1 antibody (Cat#63805, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Crystallin zeta like 1 expression in HeLa cells using Crystallin zeta like 1 antibody (Cat#63805, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Crystallin zeta like 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CRYZL1
  • Catalog：
    63805
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated CRYZL1 Mouse mAb
Aliases CRYZL1; Crystallin Zeta Like 1; QOH-1; 4P11; FERRY4; Fy-4; Crystallin, Zeta (Quinone Reductase)-Like 1; Quinone Oxidoreductase-Like Protein 1; Quinone Oxidoreductase Homolog 1; Quinone Reductase-Like 1; Zeta-Crystallin Homolog; Protein 4P11; Ferry Endosomal RAB5 Effector Complex Subunit 4; EC 1.-.-.-
Background

Gene Name: CRYZL1

NCBI Gene Entry: 9946

UniProt Entry: O95825

Application Information

Molecular Weight: Predicted, 39 kDa; observed, 42 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB7960

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human CRYZL1
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using crystallin zeta like 1 antibody (Cat#63805). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with crystallin zeta like 1 antibody (Cat#63805, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
  • Western blotting analysis using crystallin zeta like 1 antibody (Cat#63805). Crystallin zeta like 1 expression in wild-type (WT) and crystallin zeta like 1 (CRYZL1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with crystallin zeta like 1 antibody (Cat#63805, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of Crystallin zeta like 1 expression in HeLa cells using Crystallin zeta like 1 antibody (Cat#63805, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Crystallin zeta like 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CRYZL1
  • Catalog：
    63805
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using crystallin zeta like 1 antibody (Cat#63805). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with crystallin zeta like 1 antibody (Cat#63805, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Western blotting analysis using crystallin zeta like 1 antibody (Cat#63805). Crystallin zeta like 1 expression in wild-type (WT) and crystallin zeta like 1 (CRYZL1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with crystallin zeta like 1 antibody (Cat#63805, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Crystallin zeta like 1 expression in HeLa cells using Crystallin zeta like 1 antibody (Cat#63805, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Crystallin zeta like 1.
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