Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

KD-Validated ATP5C1 Mouse mAb

#63811

Click to copy product information

Return to List

shRNA Knockdown Validated
Western blotting analysis using ATP5C1 antibody (Cat#63811). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP5C1 antibody (Cat#63811, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ATP5C1 antibody (Cat#63811). ATP5C1 expression in wild type (WT) and ATP5C1(ATP5F1C) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATP5C1 antibody (Cat#63811, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using ATP5C1 antibody (Cat#63811, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with ATP5C1 antibody (Cat#63811, 1:500). Nuclei were stained blue with DAPI; ATP5C1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar, 20 μm.
Immunocytochemical staining of HT-1080 cells using ATP5C1 antibody (Cat#63811, 1:1,000), Top panel: wild-type (WT); Bottom panal: ATP5C1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; ATP5C1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar, 20 μm.
Flow cytometric analysis of ATP5C1 expression in HepG2 cells using ATP5C1 antibody (Cat#63811, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATP5C1.
Validation of ATP5C1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with ATP5C1 antibody (Cat#63811, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP5F1C
  • Catalog：
    63811
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated ATP5C1 Mouse mAb
Aliases ATP5F1C; ATP Synthase F1 Subunit Gamma; ATP5CL1; ATP5C1; ATP5C; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Gamma Polypeptide 1; ATP Synthase Subunit Gamma, Mitochondrial; F-ATPase Gamma Subunit; Mitochondrial ATP Synthase, Gamma Subunit 1; ATP Synthase Gamma Chain, Mitochondrial
Background

Gene Name: ATP5F1C

NCBI Gene Entry: 509

UniProt Entry: P36542

Application Information

Molecular Weight: Predicted, 33 kDa; observed, 33 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB7990

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen Recombinant protein of human ATP5C1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:500
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using ATP5C1 antibody (Cat#63811). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP5C1 antibody (Cat#63811, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using ATP5C1 antibody (Cat#63811). ATP5C1 expression in wild type (WT) and ATP5C1(ATP5F1C) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATP5C1 antibody (Cat#63811, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using ATP5C1 antibody (Cat#63811, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HepG2 cells with ATP5C1 antibody (Cat#63811, 1:500). Nuclei were stained blue with DAPI; ATP5C1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar, 20 μm.
  • Immunocytochemical staining of HT-1080 cells using ATP5C1 antibody (Cat#63811, 1:1,000), Top panel: wild-type (WT); Bottom panal: ATP5C1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; ATP5C1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar, 20 μm.
  • Flow cytometric analysis of ATP5C1 expression in HepG2 cells using ATP5C1 antibody (Cat#63811, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATP5C1.
  • Validation of ATP5C1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with ATP5C1 antibody (Cat#63811, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP5F1C
  • Catalog：
    63811
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using ATP5C1 antibody (Cat#63811). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP5C1 antibody (Cat#63811, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ATP5C1 antibody (Cat#63811). ATP5C1 expression in wild type (WT) and ATP5C1(ATP5F1C) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATP5C1 antibody (Cat#63811, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using ATP5C1 antibody (Cat#63811, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with ATP5C1 antibody (Cat#63811, 1:500). Nuclei were stained blue with DAPI; ATP5C1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar, 20 μm.
Immunocytochemical staining of HT-1080 cells using ATP5C1 antibody (Cat#63811, 1:1,000), Top panel: wild-type (WT); Bottom panal: ATP5C1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; ATP5C1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar, 20 μm.
Flow cytometric analysis of ATP5C1 expression in HepG2 cells using ATP5C1 antibody (Cat#63811, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATP5C1.
Validation of ATP5C1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with ATP5C1 antibody (Cat#63811, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.