Helicase like transcription factor Mouse mAb

#6466

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Western blotting analysis using HLTF antibody (Cat#6466). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLTF antibody (Cat#6466, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Immunocytochemical staining of MCF-7 cells with HLTF antibody (Cat#6466, 1:1,000). Nuclei were stained blue with DAPI; HLTF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.

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Specification：

10 μL 20 μL 100 μL

Unit price：

$69.00

Quantity：

(Excluding sales tax)

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Gene Symbol：

HLTF
Catalog：

6466
Application：

WB, ICC
Species Reactivity：

H

Information

Data

Information

Product Name	Helicase like transcription factor Mouse mAb
Aliases	HLTF; Helicase Like Transcription Factor; HIP116A; RNF80; SMARCA3; SNF2L3; HLTF1; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 3; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 3; RING-Type E3 Ubiquitin Transferase HLTF; Sucrose Nonfermenting Protein 2-Like 3; Helicase-Like Transcription Factor; RING Finger Protein 80; HIP116; ZBU1; DNA-Binding Protein/Plasminogen Activator Inhibitor-1 Regulator; DNA-Binding Protein/Plasminogen Activator Inhibitor 1 Regulator; Sucrose Nonfermenting-Like 3; SNF2-Like 3; EC 2.3.2.27; EC 3.6.4.-; EC 3.6.1
Background	Gene Name: HLTF NCBI Gene Entry: 6596 UniProt Entry: Q14527
Application Information	Molecular Weight: Predicted, 114 kDa; observed, 114 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB9225 Species Reactivity: Human Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen	Recombinant protein of human HLTF
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:400-1:2,000 Immunocytochemistry (ICC): 1:100-1:1,000
Note	This product is for research use only.

Data

Western blotting analysis using HLTF antibody (Cat#6466). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLTF antibody (Cat#6466, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of MCF-7 cells with HLTF antibody (Cat#6466, 1:1,000). Nuclei were stained blue with DAPI; HLTF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$69.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

HLTF
Catalog：

6466
Application：

WB, ICC
Species Reactivity：

H

Datasheet

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