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ITGA2 Mouse mAb

#6473

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Western blotting analysis using ITGA2 antibody (Cat#6473). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ITGA2 antibody (Cat#6473, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of ITGA2 expression in HT-1080 cells using ITGA2 antibody (Cat#6473, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ITGA2.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    ITGA2
  • Catalog:
    6473
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name ITGA2 Mouse mAb
Aliases ITGA2; Integrin Subunit Alpha 2; GPIa; VLAA2; HPA-5; CD49B; Integrin, Alpha 2 (CD49B, Alpha 2 Subunit Of VLA-2 Receptor); Very Late Activation Receptor Subunit Alpha-2; Alpha 2 Subunit Of VLA-2 Receptor; CD49 Antigen-Like Family Member B; Platelet Membrane Glycoprotein Ia; Human Platelet Alloantigen 5; Collagen Receptor; Alpha 2 Integrin; Integrin Alpha-2; Glycoprotein Ia; Very Late Activation Protein 2 Receptor, Alpha-2 Subunit; Very Late Activation Receptor Alpha-2 Subunit; Human Platelet Alloantigen System 5; Platelet Glycoprotein GPIa; Platelet Antigen Br; VLA-2 Subunit Alpha; CD49b Antigen; VLA-2; BR
Background

Gene Name: ITGA2

NCBI Gene Entry: 3673

UniProt Entry: P17301

Application Information

Molecular Weight: Predicted, 129 kDa; observed, 150 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9260

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human ITGA2
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using ITGA2 antibody (Cat#6473). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ITGA2 antibody (Cat#6473, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of ITGA2 expression in HT-1080 cells using ITGA2 antibody (Cat#6473, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ITGA2.
Western blotting analysis using ITGA2 antibody (Cat#6473). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ITGA2 antibody (Cat#6473, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of ITGA2 expression in HT-1080 cells using ITGA2 antibody (Cat#6473, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ITGA2.
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