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Home>Product>Primary Antibody

KD-Validated TOP2A Rabbit pAb

#64970

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shRNA Knockdown Validated
Western blotting analysis using TOP2A antibody (Cat#64970). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TOP2A antibody (Cat#64970, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using TOP2A antibody (Cat#64970). TOP2A expression in wild-type (WT) and TOP2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TOP2A antibody (Cat#64970, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TOP2A
  • Catalog：
    64970
  • Application：
    WB
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name KD-Validated TOP2A Rabbit pAb
Aliases TOP2A; DNA Topoisomerase II Alpha; TOP2alpha; TOPIIA; TOP2; Topoisomerase (DNA) II Alpha 170kDa; DNA Topoisomerase II, Alpha Isozyme; DNA Topoisomerase 2-Alpha; DNA Topoisomerase (ATP-Hydrolyzing); DNA Topoisomerase II, 170 KD; EC 5.99.1.3; DNA Gyrase; EC 5.6.2.2; TP2A
Background

Gene Name: TOP2A

NCBI Gene Entry: 7153

UniProt Entry: P11388

Application Information

Molecular Weight: Predicted, 174 kDa; observed, 190 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human Topoisomerase 2 alpha
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Data
  • Western blotting analysis using TOP2A antibody (Cat#64970). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TOP2A antibody (Cat#64970, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using TOP2A antibody (Cat#64970). TOP2A expression in wild-type (WT) and TOP2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TOP2A antibody (Cat#64970, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TOP2A
  • Catalog：
    64970
  • Application：
    WB
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using TOP2A antibody (Cat#64970). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TOP2A antibody (Cat#64970, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using TOP2A antibody (Cat#64970). TOP2A expression in wild-type (WT) and TOP2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TOP2A antibody (Cat#64970, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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