KD-Validated MAPRE2 Mouse mAb - Product - Hefei GenuIN Biotech Co., Ltd.

KD-Validated MAPRE2 Mouse mAb

#65036

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Knockdown Validated

Western blotting analysis using MAPRE2 antibody (Cat#65036). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPRE2 antibody (Cat#65036, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using MAPRE2 antibody (Cat#65036). MAPRE2 expression in wild-type (WT) and MAPRE2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAPRE2 antibody (Cat#65036, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of MAPRE2 expression in HeLa cells using MAPRE2 antibody (Cat#65036, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, MAPRE2.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

MAPRE2
Catalog：

65036
Application：

WB, FCM
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KD-Validated MAPRE2 Mouse mAb
Aliases	MAPRE2; Microtubule Associated Protein RP/EB Family Member 2; RP1; EB2; EB1; Microtubule-Associated Protein RP/EB Family Member 2; APC-Binding Protein EB1; APC-Binding Protein EB2; End-Binding Protein 2; T-Cell Activation Protein, EB1 Family; CSCSC2
Background	Gene Name: MAPRE2 NCBI Gene Entry: 10982 UniProt Entry: Q15555
Application Information	Molecular Weight: Predicted, 37 kDa; observed, 37 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB13965 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human MAPRE2
Isotype	Mouse IgG2a
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:100-1:1,000
Note	This product is for research use only.

Data

Western blotting analysis using MAPRE2 antibody (Cat#65036). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPRE2 antibody (Cat#65036, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using MAPRE2 antibody (Cat#65036). MAPRE2 expression in wild-type (WT) and MAPRE2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAPRE2 antibody (Cat#65036, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of MAPRE2 expression in HeLa cells using MAPRE2 antibody (Cat#65036, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, MAPRE2.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

MAPRE2
Catalog：

65036
Application：

WB, FCM
Species Reactivity：

H, M, R

Datasheet

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