Western blotting analysis using phosducin like 3 antibody (Cat#65059). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phosducin like 3 antibody (Cat#65059, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using phosducin like 3 antibody (Cat#65059). Phosducin like 3 expression in wild-type (WT) and phosducin like 3 (PDCL3) knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phosducin like 3 antibody (Cat#65059, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Phosducin like 3 expression in HeLa cells using Phosducin like 3 antibody (Cat#65059, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phosducin like 3.
