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Home>Product>Primary Antibody

KD-Validated IMMT Mouse mAb

#65108

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shRNA Knockdown Validated
Western blotting analysis using IMMT antibody (Cat#65108). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IMMT antibody (Cat#65108, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using IMMT antibody (Cat#65108). IMMT expression in wild-type (WT) and IMMT shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IMMT antibody (Cat#65108, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with IMMT antibody (Cat#65108, 1:1,000). Nuclei were stained blue with DAPI; IMMT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of IMMT expression in HepG2 cells using IMMT antibody (Cat#65108, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, IMMT.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    IMMT
  • Catalog：
    65108
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated IMMT Mouse mAb
Aliases IMMT; Inner Membrane Mitochondrial Protein; MINOS2; HMP; Mitofilin; MICOS60; Mic60; P87; P89; Mitochondrial Contact Site And Cristae Organizing System Subunit 60; Mitochondrial Inner Membrane Organizing System 2; Cell Proliferation-Inducing Gene 4/52 Protein; Mitochondrial Inner Membrane Protein; MICOS Complex Subunit MIC60; Heart Muscle Protein; P87/89; Inner Membrane Protein, Mitochondrial (Mitofilin); Cell Proliferation-Inducing Protein 52; Proliferation-Inducing Gene 4; Motor Protein; PIG52; MIC60; PIG4
Background

Gene Name: IMMT

NCBI Gene Entry: 10989

UniProt Entry: Q16891

Application Information

Molecular Weight: Predicted, 84 kDa; observed, 75 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB14355

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human IMMT
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:100-1:1,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using IMMT antibody (Cat#65108). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IMMT antibody (Cat#65108, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using IMMT antibody (Cat#65108). IMMT expression in wild-type (WT) and IMMT shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IMMT antibody (Cat#65108, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with IMMT antibody (Cat#65108, 1:1,000). Nuclei were stained blue with DAPI; IMMT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of IMMT expression in HepG2 cells using IMMT antibody (Cat#65108, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, IMMT.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    IMMT
  • Catalog：
    65108
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using IMMT antibody (Cat#65108). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IMMT antibody (Cat#65108, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using IMMT antibody (Cat#65108). IMMT expression in wild-type (WT) and IMMT shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IMMT antibody (Cat#65108, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with IMMT antibody (Cat#65108, 1:1,000). Nuclei were stained blue with DAPI; IMMT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of IMMT expression in HepG2 cells using IMMT antibody (Cat#65108, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, IMMT.
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