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Home>Product>Primary Antibody

HSPA1A Mouse mAb

#6575

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Western blotting analysis using HSPA1A antibody (Cat#6575). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPA1A antibody (Cat#6575, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using HSPA1A antibody (Cat#6575). Total cell lysates (30 μg) from H9c2 and C2C12 cells were loaded and separated by SDS-PAGE. The blot was incubated with HSPA1A antibody (Cat#6575, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with HSPA1A antibody (Cat#6575, 1:1,000). Nuclei were stained blue with DAPI; HSPA1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HSPA1A expression in HepG2 cells using HSPA1A antibody (Cat#6575, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HSPA1A.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HSPA1A
  • Catalog：
    6575
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name HSPA1A Mouse mAb
Aliases HSPA1A; Heat Shock Protein Family A (Hsp70) Member 1A; HSP70-1; HSPA1; Heat Shock Protein Family A Member 1A; Heat Shock 70 KDa Protein 1A; Heat Shock 70kDa Protein 1A; Heat Shock 70 KDa Protein 1; Heat Shock 70kD Protein 1A; HSP70.1; HSP72; Epididymis Secretory Sperm Binding Protein; DnaK-Type Molecular Chaperone HSP70-1; Epididymis Secretory Protein Li 103; Heat Shock 70 KDa Protein 1A/1B; Heat Shock 70 KDa Protein 1/2; Heat Shock 70 KDa Protein 1B; Heat Shock 70 KDa Protein 2; Heat Shock-Induced Protein; HSP70-1/HSP70-2; HSP70.1/HSP70.2; HEL-S-103; HSP70-1A; HSP70-2; HSP70.2; HSP70I; HSP70; HSX70
Background

Gene Name: HSPA1A

NCBI Gene Entry: 3303

UniProt Entry: P0DMV8

Application Information

Molecular Weight: Predicted, 70 kDa; observed, 70 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9620

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human HSPA1A
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using HSPA1A antibody (Cat#6575). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPA1A antibody (Cat#6575, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using HSPA1A antibody (Cat#6575). Total cell lysates (30 μg) from H9c2 and C2C12 cells were loaded and separated by SDS-PAGE. The blot was incubated with HSPA1A antibody (Cat#6575, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with HSPA1A antibody (Cat#6575, 1:1,000). Nuclei were stained blue with DAPI; HSPA1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of HSPA1A expression in HepG2 cells using HSPA1A antibody (Cat#6575, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HSPA1A.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HSPA1A
  • Catalog：
    6575
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using HSPA1A antibody (Cat#6575). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPA1A antibody (Cat#6575, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using HSPA1A antibody (Cat#6575). Total cell lysates (30 μg) from H9c2 and C2C12 cells were loaded and separated by SDS-PAGE. The blot was incubated with HSPA1A antibody (Cat#6575, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with HSPA1A antibody (Cat#6575, 1:1,000). Nuclei were stained blue with DAPI; HSPA1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HSPA1A expression in HepG2 cells using HSPA1A antibody (Cat#6575, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HSPA1A.
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