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Home>Product>Primary Antibody

KD-Validated HSD17B4 Mouse mAb

#65880

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shRNA Knockdown Validated
Western blotting analysis using HSD17B4 antibody (Cat#65880). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSD17B4 antibody (Cat#65880, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using HSD17B4 antibody (Cat#65880). HSD17B4 expression in wild-type (WT) and HSD17B4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HSD17B4 antibody (Cat#65880, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using HSD17B4 antibody (Cat#65880, 1:1,000), Top panel: wild-type (WT); Bottom panal: HSD17B4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; HSD17B4 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HepG2 cells with HSD17B4 antibody (Cat#65880, 1:1,000). Nuclei were stained blue with DAPI; HSD17B4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of HSD17B4 expression in HepG2 cells using HSD17B4 antibody (Cat#65880,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HSD17B4.
Validation of HSD17B4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HSD17B4 antibody (Cat#65880, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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  • Gene Symbol：
    HSD17B4
  • Catalog：
    65880
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name KD-Validated HSD17B4 Mouse mAb
Aliases HSD17B4; Hydroxysteroid 17-Beta Dehydrogenase 4; SDR8C1; MFE-2; DBP; 17-Beta-Hydroxysteroid Dehydrogenase 4; 3-Alpha,7-Alpha,12-Alpha-Trihydroxy-5-Beta-Cholest-24-Enoyl-CoA Hydratase; Short Chain Dehydrogenase/Reductase Family 8C Member 1; Peroxisomal Multifunctional Enzyme Type 2; 17beta-Estradiol Dehydrogenase Type IV; Peroxisomal Multifunctional Protein 2; D-Bifunctional Protein, Peroxisomal; D-3-Hydroxyacyl-CoA Dehydratase; Beta-Hydroxyacyl Dehydrogenase; Multifunctional Protein 2; Beta-Keto-Reductase; 17-Beta-HSD IV; 17-Beta-HSD 4; MFP-2; Short Chain Dehydrogenase/Reductase Family 8C, Member 1; Epididymis Secretory Sperm Binding Protein; Hydroxysteroid (17-Beta) Dehydrogenase 4; Hydroxysteroid Dehydrogenase 4; D-Bifunctional Protein; EDH17B4; PRLTS1; MPF-2
Background

Gene Name: HSD17B4

NCBI Gene Entry: 3295

UniProt Entry: P51659

Application Information

Molecular Weight: Predicted, 80 kDa; observed, 45,73 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5635

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human HSD17B4
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using HSD17B4 antibody (Cat#65880). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSD17B4 antibody (Cat#65880, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using HSD17B4 antibody (Cat#65880). HSD17B4 expression in wild-type (WT) and HSD17B4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HSD17B4 antibody (Cat#65880, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HeLa cells using HSD17B4 antibody (Cat#65880, 1:1,000), Top panel: wild-type (WT); Bottom panal: HSD17B4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; HSD17B4 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Immunocytochemical staining of HepG2 cells with HSD17B4 antibody (Cat#65880, 1:1,000). Nuclei were stained blue with DAPI; HSD17B4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of HSD17B4 expression in HepG2 cells using HSD17B4 antibody (Cat#65880,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HSD17B4.
  • Validation of HSD17B4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HSD17B4 antibody (Cat#65880, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HSD17B4
  • Catalog：
    65880
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using HSD17B4 antibody (Cat#65880). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSD17B4 antibody (Cat#65880, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using HSD17B4 antibody (Cat#65880). HSD17B4 expression in wild-type (WT) and HSD17B4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HSD17B4 antibody (Cat#65880, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using HSD17B4 antibody (Cat#65880, 1:1,000), Top panel: wild-type (WT); Bottom panal: HSD17B4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; HSD17B4 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HepG2 cells with HSD17B4 antibody (Cat#65880, 1:1,000). Nuclei were stained blue with DAPI; HSD17B4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of HSD17B4 expression in HepG2 cells using HSD17B4 antibody (Cat#65880,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HSD17B4.
Validation of HSD17B4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HSD17B4 antibody (Cat#65880, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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