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Home>Product>Primary Antibody

KD-Validated PPP4C Recombinant Rabbit mAb

#66891

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using PPP4C antibody (Cat#66891). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP4C antibody (Cat#66891, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PPP4C antibody (Cat#66891). PPP4C expression in wild type (WT) and PPP4C shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PPP4C antibody (Cat#66891, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with PPP4C antibody (Cat#66891, 1:1,000). Nuclei were stained blue with DAPI; PPP4C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
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  • Gene Symbol：
    PPP4C
  • Catalog：
    66891
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated PPP4C Recombinant Rabbit mAb
Aliases PPP4C; Protein Phosphatase 4 Catalytic Subunit; PPX; PP4; Serine/Threonine-Protein Phosphatase 4 Catalytic Subunit; Protein Phosphatase 4 (Formerly X), Catalytic Subunit; Protein Phosphatase X, Catalytic Subunit; EC 3.1.3.16; PP-X; PP4C; PPP4; Epididymis Secretory Sperm Binding Protein; Protein Phosphatase 4, Catalytic Subunit; Protein Phosphatase X; PPH3; Pp4
Background

Gene Name: PPP4C

NCBI Gene Entry: 5531

UniProt Entry: P60510

Application Information

Molecular Weight: Predicted, 35 kDa; observed, 32 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB1205

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human PPP4C
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PPP4C antibody (Cat#66891). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP4C antibody (Cat#66891, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using PPP4C antibody (Cat#66891). PPP4C expression in wild type (WT) and PPP4C shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PPP4C antibody (Cat#66891, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with PPP4C antibody (Cat#66891, 1:1,000). Nuclei were stained blue with DAPI; PPP4C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PPP4C
  • Catalog：
    66891
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using PPP4C antibody (Cat#66891). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP4C antibody (Cat#66891, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PPP4C antibody (Cat#66891). PPP4C expression in wild type (WT) and PPP4C shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PPP4C antibody (Cat#66891, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with PPP4C antibody (Cat#66891, 1:1,000). Nuclei were stained blue with DAPI; PPP4C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
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