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KD-Validated CCN1 Recombinant Rabbit mAb

#67011

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using CCN1 antibody (Cat#67011). Total cell lysates (40 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CCN1 antibody (Cat#67011, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:5,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
Immunocytochemical staining of HeLa cells using CCN1 antibody (Cat#67011, 1:50). Top panel: wild type (WT); Bottom panel: CCN1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; CCN1 was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
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  • Gene Symbol:
    CCN1
  • Catalog:
    67011
  • Application:
    WB, ICC
  • Species Reactivity:
    H
Information
Product Name KD-Validated CCN1 Recombinant Rabbit mAb
Aliases CCN1; Cellular Communication Network Factor 1; GIG1; IGFBP10; CYR61; Insulin-Like Growth Factor-Binding Protein 10; Cysteine Rich Angiogenic Inducer 61; IGF-Binding Protein 10; CCN Family Member 1; Protein CYR61; IGFBP-10; IBP-10; Cysteine-Rich Heparin-Binding Protein 61; Cysteine-Rich, Anigogenic Inducer, 61; Cysteine-Rich Angiogenic Inducer 61; Protein GIG1
Background

Gene Name: CCN1

NCBI Gene Entry: 3491

UniProt Entry: O00622

Application Information

Molecular Weight: Predicted, 42 kDa; observed, 41 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB2380

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human CCN1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000
Immunocytochemistry (ICC): 1:50
Note This product is for research use only.
Data
  • Western blotting analysis using CCN1 antibody (Cat#67011). Total cell lysates (40 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CCN1 antibody (Cat#67011, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:5,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
  • Immunocytochemical staining of HeLa cells using CCN1 antibody (Cat#67011, 1:50). Top panel: wild type (WT); Bottom panel: CCN1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; CCN1 was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
Western blotting analysis using CCN1 antibody (Cat#67011). Total cell lysates (40 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CCN1 antibody (Cat#67011, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:5,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
Immunocytochemical staining of HeLa cells using CCN1 antibody (Cat#67011, 1:50). Top panel: wild type (WT); Bottom panel: CCN1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; CCN1 was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
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