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Home>Product>Primary Antibody

KD-Validated NUDC Mouse mAb

#66446

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shRNA Knockdown Validated
Western blotting analysis using NUDC antibody (Cat#66446). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NUDC antibody (Cat#66446, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using NUDC antibody (Cat#66446). NUDC expression in wild-type (WT) and NUDC shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NUDC antibody (Cat#66446, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of NUDC expression in HepG2 cells using NUDC antibody (Cat#66446, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NUDC.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
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  • Gene Symbol：
    NUDC
  • Catalog：
    66446
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated NUDC Mouse mAb
Aliases NUDC; Nuclear Distribution C, Dynein Complex Regulator; Nuclear Distribution Protein C Homolog; NudC Nuclear Distribution Protein; Nuclear Migration Protein NudC; Nuclear Distribution Gene C Homolog (A. Nidulans); Nuclear Distribution C Homolog (A. Nidulans); Nuclear Distribution Gene C Homolog; Nuclear Distribution C Homolog; NPD011; HNUDC; MNUDC
Background

Gene Name: NUDC

NCBI Gene Entry: 10726

UniProt Entry: Q9Y266

Application Information

Molecular Weight: Predicted, 38 kDa; observed, 42 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9135

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human NUDC
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using NUDC antibody (Cat#66446). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NUDC antibody (Cat#66446, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using NUDC antibody (Cat#66446). NUDC expression in wild-type (WT) and NUDC shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NUDC antibody (Cat#66446, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of NUDC expression in HepG2 cells using NUDC antibody (Cat#66446, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NUDC.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NUDC
  • Catalog：
    66446
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using NUDC antibody (Cat#66446). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NUDC antibody (Cat#66446, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using NUDC antibody (Cat#66446). NUDC expression in wild-type (WT) and NUDC shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NUDC antibody (Cat#66446, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of NUDC expression in HepG2 cells using NUDC antibody (Cat#66446, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NUDC.
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