Western blotting analysis using ENOPH1 antibody (Cat#61869). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ENOPH1 antibody (Cat#61869, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ENOPH1 antibody (Cat#61869). ENOPH1 expression in wild type (WT) and ENOPH1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ENOPH1 antibody (Cat#61869, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ENOPH1 expression in HAP-1 cells using ENOPH1 antibody (Cat#61869, 1:1,000). Green, isotype control; red, ENOPH1.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human ENOPH1
Isotype
Mouse IgG2a
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using ENOPH1 antibody (Cat#61869). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ENOPH1 antibody (Cat#61869, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ENOPH1 antibody (Cat#61869). ENOPH1 expression in wild type (WT) and ENOPH1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ENOPH1 antibody (Cat#61869, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ENOPH1 expression in HAP-1 cells using ENOPH1 antibody (Cat#61869, 1:1,000). Green, isotype control; red, ENOPH1.
Western blotting analysis using ENOPH1 antibody (Cat#61869). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ENOPH1 antibody (Cat#61869, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ENOPH1 antibody (Cat#61869). ENOPH1 expression in wild type (WT) and ENOPH1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ENOPH1 antibody (Cat#61869, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ENOPH1 expression in HAP-1 cells using ENOPH1 antibody (Cat#61869, 1:1,000). Green, isotype control; red, ENOPH1.
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