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KD-Validated JAK2 Recombinant Rabbit mAb

#67020

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Recombinant Antibody
shRNA Knockdown Validated
Immunocytochemical staining of HeLa cells using JAK2 antibody (Cat#67020, 1:100). Top panel: wild type (WT); Bottom panel: JAK2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; actin was stained green with Alexa Fluor 488; JAK2 was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
Flow cytometric analysis of JAK2 expression in HeLa cells using JAK2 antibody (Cat#67020, 1:1,000). Blue: wild type (WT); red: JAK2 shRNA knockdown (KD).
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  • Gene Symbol:
    JAK2
  • Catalog:
    67020
  • Application:
    WB, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name KD-Validated JAK2 Recombinant Rabbit mAb
Aliases JAK2; Janus Kinase 2; JTK10; Tyrosine-Protein Kinase JAK2; EC 2.7.10.2; JAK-2; Janus Kinase 2 (A Protein Tyrosine Kinase); EC 2.7.10
Background

Gene Name: JAK2

NCBI Gene Entry: 3717

UniProt Entry: O60674

Application Information

Molecular Weight: Predicted, 131 kDa; observed, 125 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB2420

Species Reactivity: Human, mouse, rat

Applications Tested: Flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human JAK2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Flow cytometry (FCM): 1:50
Immunocytochemistry (ICC): 1:100
Note This product is for research use only.
Data
  • Immunocytochemical staining of HeLa cells using JAK2 antibody (Cat#67020, 1:100). Top panel: wild type (WT); Bottom panel: JAK2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; actin was stained green with Alexa Fluor 488; JAK2 was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
  • Flow cytometric analysis of JAK2 expression in HeLa cells using JAK2 antibody (Cat#67020, 1:1,000). Blue: wild type (WT); red: JAK2 shRNA knockdown (KD).
Immunocytochemical staining of HeLa cells using JAK2 antibody (Cat#67020, 1:100). Top panel: wild type (WT); Bottom panel: JAK2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; actin was stained green with Alexa Fluor 488; JAK2 was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
Flow cytometric analysis of JAK2 expression in HeLa cells using JAK2 antibody (Cat#67020, 1:1,000). Blue: wild type (WT); red: JAK2 shRNA knockdown (KD).
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