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KD-Validated RARG Recombinant Rabbit mAb

#67031

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using RARG antibody (Cat#67031, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using RARG antibody (Cat#67031, 1:1,000). RARG expression in wild-type (WT) and RARG shRNA knockdown (KD) MCF7 cells of total cell lysates. Hsp60 serves as a loading control.
Immunocytochemical staining of MCF7 cells using RARG antibody (Cat#67031, 1:200). Top panel: wild type (WT); Bottom panel: RARG shRNA knockdown (KD). Nuclei were stained blue with DAPI; actin was stained green with Alexa Fluor 488; RARG was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
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  • Gene Symbol:
    RARG
  • Catalog:
    67031
  • Application:
    WB, ICC
  • Species Reactivity:
    H, M
Information
Product Name KD-Validated RARG Recombinant Rabbit mAb
Aliases RARG; Retinoic Acid Receptor Gamma; RAR-Gamma; NR1B3; RARgamma; RARC; Nuclear Receptor Subfamily 1 Group B Member 3; Retinoic Acid Receptor, Gamma
Background

Gene Name: RARG

NCBI Gene Entry: 5916

UniProt Entry: P13631

Application Information

Molecular Weight: Predicted, 50 kDa; observed, 58 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB2475

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human RARG
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000
Immunocytochemistry (ICC): 1:200
Note This product is for research use only.
Data
  • Western blotting analysis using RARG antibody (Cat#67031, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
  • Western blotting analysis using RARG antibody (Cat#67031, 1:1,000). RARG expression in wild-type (WT) and RARG shRNA knockdown (KD) MCF7 cells of total cell lysates. Hsp60 serves as a loading control.
  • Immunocytochemical staining of MCF7 cells using RARG antibody (Cat#67031, 1:200). Top panel: wild type (WT); Bottom panel: RARG shRNA knockdown (KD). Nuclei were stained blue with DAPI; actin was stained green with Alexa Fluor 488; RARG was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
Western blotting analysis using RARG antibody (Cat#67031, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using RARG antibody (Cat#67031, 1:1,000). RARG expression in wild-type (WT) and RARG shRNA knockdown (KD) MCF7 cells of total cell lysates. Hsp60 serves as a loading control.
Immunocytochemical staining of MCF7 cells using RARG antibody (Cat#67031, 1:200). Top panel: wild type (WT); Bottom panel: RARG shRNA knockdown (KD). Nuclei were stained blue with DAPI; actin was stained green with Alexa Fluor 488; RARG was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
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