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Myosin Light Chain 9 Recombinant Rabbit mAb

#2380

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Recombinant Antibody
Western blotting analysis using Myosin light chain 9 antibody (Cat#2380). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Myosin light chain 9 antibody (Cat#2380, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Myosin light chain 9 expression in H9c2 cells using Myosin light chain 9 antibody (Cat#2380, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Myosin light chain 9.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    MYL9
  • Catalog:
    2380
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name Myosin Light Chain 9 Recombinant Rabbit mAb
Aliases Myosin Light Chain 9; MYRL2; MRLC1; MLC2; LC20; Myosin Regulatory Light Chain 2, Smooth Muscle Isoform; Myosin, Light Polypeptide 9, Regulatory; Myosin Regulatory Light Polypeptide 9; Myosin Regulatory Light Chain MRLC1; Myosin, Light Chain 9, Regulatory; Myosin Regulatory Light Chain 1; Myosin Regulatory Light Chain 9; 20 KDa Myosin Light Chain; Myosin RLC; MLC-2C; Epididymis Secretory Sperm Binding Protein; MMIHS4
Background

Gene Name: MYL9

NCBI Gene Entry: 10398

UniProt Entry: P24844

Application Information

Molecular Weight: Predicted, 20 kDa; observed, 18 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB565

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human MYL9
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using Myosin light chain 9 antibody (Cat#2380). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Myosin light chain 9 antibody (Cat#2380, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Myosin light chain 9 expression in H9c2 cells using Myosin light chain 9 antibody (Cat#2380, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Myosin light chain 9.
Western blotting analysis using Myosin light chain 9 antibody (Cat#2380). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Myosin light chain 9 antibody (Cat#2380, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Myosin light chain 9 expression in H9c2 cells using Myosin light chain 9 antibody (Cat#2380, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Myosin light chain 9.
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