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Home>Product>Primary Antibody

CD80 Recombinant Rabbit mAb

#6894

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Recombinant Antibody
Western blotting analysis using CD80 antibody (Cat#6894). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD80 antibody (Cat#6894, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of raji cells with CD80 antibody (Cat#6894, 1:1,000). Nuclei were stained blue with DAPI; CD80 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CD80
  • Catalog：
    6894
  • Application：
    WB, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name CD80 Recombinant Rabbit mAb
Aliases CD80; CD80 Molecule; CD28LG1; CD28LG; B7.1; B7-1; CD80 Antigen (CD28 Antigen Ligand 1, B7-1 Antigen); T-Lymphocyte Activation Antigen CD80; B-Lymphocyte Activation Antigen B7; CTLA-4 Counter-Receptor B7.1; Activation B7-1 Antigen; LAB7; BB1; B7; Costimulatory Molecule Variant IgV-CD80; Costimulatory Factor CD80; CD80 Molecule; CD80 Antigen
Background

Gene Name: CD80

NCBI Gene Entry: 941

UniProt Entry: P33681

Application Information

Molecular Weight: Predicted, 33 kDa; observed, 50-75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB1220

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human CD80
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CD80 antibody (Cat#6894). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD80 antibody (Cat#6894, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of raji cells with CD80 antibody (Cat#6894, 1:1,000). Nuclei were stained blue with DAPI; CD80 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CD80
  • Catalog：
    6894
  • Application：
    WB, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using CD80 antibody (Cat#6894). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD80 antibody (Cat#6894, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of raji cells with CD80 antibody (Cat#6894, 1:1,000). Nuclei were stained blue with DAPI; CD80 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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