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Home>Product>Primary Antibody

PTK2 Recombinant Mouse mAb

#6788

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Recombinant Antibody
Western blotting analysis using PTK2 antibody (Cat#6788). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PTK2 antibody (Cat#6788, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with PTK2 antibody (Cat#6788, 1:1,000). Nuclei were stained blue with DAPI; PTK2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PTK2 expression in HAP-1 cells using PTK2 antibody (Cat#6788, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PTK2
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PTK2
  • Catalog：
    6788
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name PTK2 Recombinant Mouse mAb
Aliases PTK2; Protein Tyrosine Kinase 2; PPP1R71; FAK1; FAK; FADK; Protein Phosphatase 1 Regulatory Subunit 71; Focal Adhesion Kinase-Related Nonkinase; PTK2 Protein Tyrosine Kinase 2; Focal Adhesion Kinase 1; EC 2.7.10.2; Pp125FAK; P125FAK; FADK 1; FRNK; Protein Phosphatase 1, Regulatory Subunit 71; FAK-Related Non-Kinase Polypeptide; Protein-Tyrosine Kinase 2; EC 2.7.10
Background

Gene Name: PTK2

NCBI Gene Entry: 5747

UniProt Entry: Q05397

Application Information

Molecular Weight: Predicted, 119 kDa; observed, 110 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB840

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human PTK2
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PTK2 antibody (Cat#6788). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PTK2 antibody (Cat#6788, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with PTK2 antibody (Cat#6788, 1:1,000). Nuclei were stained blue with DAPI; PTK2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of PTK2 expression in HAP-1 cells using PTK2 antibody (Cat#6788, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PTK2
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PTK2
  • Catalog：
    6788
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using PTK2 antibody (Cat#6788). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PTK2 antibody (Cat#6788, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with PTK2 antibody (Cat#6788, 1:1,000). Nuclei were stained blue with DAPI; PTK2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PTK2 expression in HAP-1 cells using PTK2 antibody (Cat#6788, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PTK2
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