Western blotting analysis using MYH9 antibody (Cat#65082). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MYH9 antibody (Cat#65082, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using MYH9 antibody (Cat#65082). MYH9 expression in wild-type (WT) and MYH9 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MYH9 antibody (Cat#65082, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of MYH9 expression in HepG2 cells using MYH9 antibody (Cat#65082, 1:1,000) . Green, isotype control; red, MYH9.
Validation of MYH9 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with MYH9 antibody (Cat#65082, 1:2,000) and analyzed using BD flow cytometer.
Immunohistochemistry was performed on paraffin-embedded human renal carcinoma using MYH9 antibody (Cat#65082, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
