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Home>Product>Primary Antibody

MEF2C/ MEF2A Recombinant Rabbit mAb

#2532

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Recombinant Antibody
Western blotting analysis using MEF2A+MEF2C antibody (Cat#2532). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MEF2A+MEF2C antibody (Cat#2532, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9c2 cells with MEF2A+MEF2C antibody (Cat#2532, 1:1,000). Nuclei were stained blue with DAPI; MEF2A+MEF2C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MEF2A+MEF2C expression in H9c2 cells using MEF2A+MEF2C antibody (Cat#2532, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, MEF2A+MEF2C.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MEF2C/ MEF2A
  • Catalog：
    2532
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name MEF2C/ MEF2A Recombinant Rabbit mAb
Aliases MEF2C; Myocyte Enhancer Factor 2C; Myocyte-Specific Enhancer Factor 2C; MADS Box Transcription Enhancer Factor 2, Polypeptide C; C5DELq14.3; DEL5q14.3; NEDHSIL; MEF2A; Myocyte Enhancer Factor 2A; RSRFC4; RSRFC9; Serum Response Factor-Like Protein 1; Myocyte-Specific Enhancer Factor 2A; MADS Box Transcription Enhancer Factor 2, Polypeptide A (Myocyte Enhancer Factor 2A); ADCAD1; Mef2; MEF2
Background

Gene Name: MEF2C/ MEF2A

NCBI Gene Entry: 4208 , 4205

UniProt Entry: Q06413 , Q02078

Application Information

Molecular Weight: Predicted, 51 kDa; observed, 51 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1280

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human MEF2A+MEF2C
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using MEF2A+MEF2C antibody (Cat#2532). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MEF2A+MEF2C antibody (Cat#2532, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of H9c2 cells with MEF2A+MEF2C antibody (Cat#2532, 1:1,000). Nuclei were stained blue with DAPI; MEF2A+MEF2C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of MEF2A+MEF2C expression in H9c2 cells using MEF2A+MEF2C antibody (Cat#2532, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, MEF2A+MEF2C.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MEF2C/ MEF2A
  • Catalog：
    2532
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using MEF2A+MEF2C antibody (Cat#2532). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MEF2A+MEF2C antibody (Cat#2532, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9c2 cells with MEF2A+MEF2C antibody (Cat#2532, 1:1,000). Nuclei were stained blue with DAPI; MEF2A+MEF2C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MEF2A+MEF2C expression in H9c2 cells using MEF2A+MEF2C antibody (Cat#2532, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, MEF2A+MEF2C.
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