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KRT19 Mouse mAb

#6789

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Western blotting analysis using KRT19 antibody (Cat#6789). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KRT19 antibody (Cat#6789, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HACAT cells with KRT19 antibody (Cat#6789, 1:1,000). Nuclei were stained blue with DAPI; KRT19 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    KRT19
  • Catalog:
    6789
  • Application:
    WB, ICC
  • Species Reactivity:
    H
Information
Product Name KRT19 Mouse mAb
Aliases KRT19; KeRin 19; K19; KeRin, Type I Cytoskeletal 19; CK19; K1CS; 40-KDa KeRin Intermediate Filament; KeRin, Type I, 40-Kd; KeRin 19, Type I; CytokeRin 19; MGC15366; CK-19; CytokeRin-19; KeRin-19
Background

Gene Name: KRT19

NCBI Gene Entry: 3880

UniProt Entry: P08727

Application Information

Molecular Weight: Predicted, 44 kDa; observed, 40 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB845

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen Recombinant protein of human KRT19
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:4,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using KRT19 antibody (Cat#6789). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KRT19 antibody (Cat#6789, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HACAT cells with KRT19 antibody (Cat#6789, 1:1,000). Nuclei were stained blue with DAPI; KRT19 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using KRT19 antibody (Cat#6789). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KRT19 antibody (Cat#6789, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HACAT cells with KRT19 antibody (Cat#6789, 1:1,000). Nuclei were stained blue with DAPI; KRT19 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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