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Home>Product>Primary Antibody

KRT5 Mouse Oligoclonal Antibody

#6808

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Western blotting analysis using KRT5 antibody (Cat#6808). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KRT5 antibody (Cat#6808, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of MCF-7 cells with KRT5 antibody (Cat#6808, 1:1,000). Nuclei were stained blue with DAPI; KRT5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    KRT5
  • Catalog：
    6808
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KRT5 Mouse Oligoclonal Antibody
Aliases KRT5; KeRin 5; CK-5; KRT5A; KeRin 5 (Epidermolysis Bullosa Simplex, Dowling-Meara/Kobner/Weber-Cockayne Types); Epidermolysis Bullosa Simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types; KeRin, Type II Cytoskeletal 5; Type-II KeRin Kb5; KeRin 5, Type II; CytokeRin-5; EBS2; K5; 58 Kda CytokeRin; 58 KDa CytokeRin; KeRin-5; EBS2A; EBS2B; EBS2C; EBS2D; EBS2E; EBS2F; DDD1; EBS1; CK5; DDD
Background

Gene Name: KRT5

NCBI Gene Entry: 3852

UniProt Entry: P13647

Application Information

Molecular Weight: Predicted, 62 kDa; observed, 50 kDa

Clonality: Mouse oligoclonal antibody

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human KRT5
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using KRT5 antibody (Cat#6808). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KRT5 antibody (Cat#6808, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of MCF-7 cells with KRT5 antibody (Cat#6808, 1:1,000). Nuclei were stained blue with DAPI; KRT5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    KRT5
  • Catalog：
    6808
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using KRT5 antibody (Cat#6808). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KRT5 antibody (Cat#6808, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of MCF-7 cells with KRT5 antibody (Cat#6808, 1:1,000). Nuclei were stained blue with DAPI; KRT5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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