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Home>Product>Primary Antibody

PKM Recombinant mAb

#6965

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Recombinant Antibody
Western blotting analysis using PKM antibody (Cat#6965). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PKM antibody (Cat#6965, 1:10,000) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:10,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with PKM antibody (Cat#6965, 1:1,000). Nuclei were stained blue with DAPI; PKM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PKM
  • Catalog：
    6965
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name PKM Recombinant mAb
Aliases PKM; Pyruvate Kinase M1/2; THBP1; OIP3; PK3; PKM2; Cytosolic Thyroid Hormone-Binding Protein; Thyroid Hormone-Binding Protein 1; Pyruvate Kinase Muscle Isozyme; Threonine-Protein Kinase PKM2; Tyrosine-Protein Kinase PKM2; Pyruvate Kinase, Muscle; Pyruvate Kinase PKM; Pyruvate Kinase 2/3; Tumor M2-PK; EC 2.7.1.40; CTHBP; P58; Thyroid Hormone-Binding Protein, Cytosolic; Epididymis Secretory Protein Li 30; Pyruvate Kinase Isozymes M1/M2; OPA-Interacting Protein 3; Opa-Interacting Protein 3; PK, Muscle Type; EC 2.7.11.1; EC 2.7.10.2; HEL-S-30; OIP-3; TCB; PK2
Background

Gene Name: PKM

NCBI Gene Entry: 5315

UniProt Entry: P14618

Application Information

Molecular Weight: Predicted, 58 kDa; observed, 58 kDa

Clonality: Human monoclonal antibody

Clone ID: 26GB2525

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human PKM
Isotype Human IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PKM antibody (Cat#6965). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PKM antibody (Cat#6965, 1:10,000) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:10,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with PKM antibody (Cat#6965, 1:1,000). Nuclei were stained blue with DAPI; PKM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PKM
  • Catalog：
    6965
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using PKM antibody (Cat#6965). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PKM antibody (Cat#6965, 1:10,000) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:10,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with PKM antibody (Cat#6965, 1:1,000). Nuclei were stained blue with DAPI; PKM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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