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Home>Product>Primary Antibody

KO-Validated Tumor Protein P53 Rabbit pAb

#71136

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Knockout Validated
Western blotting analysis using tumor protein p53 antibody (Cat#71136). Total cell lysates (30 μg) from HAP-1 cells were loaded and separated by SDS-PAGE. The blot was incubated with tumor protein p53 antibody (Cat#71136, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using tumor protein p53 antibody (Cat#71136). Tumor protein p53 expression in wild type (WT) and tumor protein p53 (TP53) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with tumor protein p53 antibody (Cat#71136, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol：
    TP53
  • Catalog：
    71136
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KO-Validated Tumor Protein P53 Rabbit pAb
Aliases TP53; Tumor Protein P53; P53; LFS1; Cellular Tumor Antigen P53; Phosphoprotein P53; Antigen NY-CO-13; Transformation-Related Protein 53; Mutant Tumor Protein 53; Li-Fraumeni Syndrome; Tumor Suppressor P53; Tumor Supressor P53; Tumor Protein 53; BMFS5; TRP53; BCC7
Background

Gene Name: TP53

NCBI Gene Entry: 7157

UniProt Entry: P04637

Application Information

Molecular Weight: Predicted, 44 kDa; observed, 50 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human p53
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using tumor protein p53 antibody (Cat#71136). Total cell lysates (30 μg) from HAP-1 cells were loaded and separated by SDS-PAGE. The blot was incubated with tumor protein p53 antibody (Cat#71136, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using tumor protein p53 antibody (Cat#71136). Tumor protein p53 expression in wild type (WT) and tumor protein p53 (TP53) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with tumor protein p53 antibody (Cat#71136, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TP53
  • Catalog：
    71136
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using tumor protein p53 antibody (Cat#71136). Total cell lysates (30 μg) from HAP-1 cells were loaded and separated by SDS-PAGE. The blot was incubated with tumor protein p53 antibody (Cat#71136, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using tumor protein p53 antibody (Cat#71136). Tumor protein p53 expression in wild type (WT) and tumor protein p53 (TP53) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with tumor protein p53 antibody (Cat#71136, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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