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Home>Product>Primary Antibody

KO-Validated DNAJB6 Mouse mAb

#71252

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Knockout Validated
Western blotting analysis using DNAJB6 antibody (Cat#71252). DNAJB6 expression in wild-type (WT) and DNAJB6 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DNAJB6 antibody (Cat#71252, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of DNAJB6 expression in HT-1080 cells using DNAJB6 antibody (Cat#71252, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DNAJB6.
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  • Gene Symbol：
    DNAJB6
  • Catalog：
    71252
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KO-Validated DNAJB6 Mouse mAb
Aliases DNAJB6; DnaJ Heat Shock Protein Family (Hsp40) Member B6; MRJ; DnaJ (Hsp40) Homolog, Subfamily B, Member 6; DnaJ Homolog Subfamily B Member 6; Heat Shock Protein J2; LGMD1D; HHDJ1; HSJ-2; MSJ-1; HSJ2; Limb Girdle Muscular Dystrophy 1D (Autosomal Dominant); DnaJ-Like 2 Protein; LGMD1E; LGMDD1; DnaJ; MSJ1; DJ4
Background

Gene Name: DNAJB6

NCBI Gene Entry: 10049

UniProt Entry: O75190

Application Information

Molecular Weight: Predicted, 36 kDa; observed, 37 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB3790

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human DNAJB6
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using DNAJB6 antibody (Cat#71252). DNAJB6 expression in wild-type (WT) and DNAJB6 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DNAJB6 antibody (Cat#71252, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of DNAJB6 expression in HT-1080 cells using DNAJB6 antibody (Cat#71252, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DNAJB6.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DNAJB6
  • Catalog：
    71252
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using DNAJB6 antibody (Cat#71252). DNAJB6 expression in wild-type (WT) and DNAJB6 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DNAJB6 antibody (Cat#71252, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of DNAJB6 expression in HT-1080 cells using DNAJB6 antibody (Cat#71252, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DNAJB6.
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