Western blotting analysis using ubiquitin C-terminal hydrolase L1 antibody (Cat#H1153). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ubiquitin C-terminal hydrolase L1 antibody (Cat#H1153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ubiquitin C-terminal hydrolase L1 antibody (Cat#H1153). Ubiquitin C-terminal hydrolase L1 expression in wild type (WT) and ubiquitin C-terminal hydrolase L1 (UCHL1) shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ubiquitin C-terminal hydrolase L1 antibody (Cat#H1153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining shows negative staining in paraffin-embedded human invasive ductal breast cancer. ubiquitin C-terminal hydrolase L1antibody (Cat#H1153) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human pancreatic cancer. ubiquitin C-terminal hydrolase L1antibody (Cat#H1153) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human ovarian cancer. ubiquitin C-terminal hydrolase L1antibody (Cat#H1153) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human pancreas. ubiquitin C-terminal hydrolase L1antibody (Cat#H1153) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human kidney. ubiquitin C-terminal hydrolase L1antibody (Cat#H1153) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human colon. ubiquitin C-terminal hydrolase L1antibody (Cat#H1153) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human cerebellum. ubiquitin C-terminal hydrolase L1antibody (Cat#H1153) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human cerebral cortex. ubiquitin C-terminal hydrolase L1antibody (Cat#H1153) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Flow cytometric analysis of Ubiquitin C-terminal hydrolase L1 expression in A549 cells using Ubiquitin C-terminal hydrolase L1 antibody (Cat#H1153, 1:2,000). Green, isotype control; red, Ubiquitin C-terminal hydrolase L1.
Validation of Ubiquitin C-terminal hydrolase L1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Ubiquitin C-terminal hydrolase L1 antibody (Cat#H1153, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
