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Home>Product>Primary Antibody

KO-Validated Placental Alkaline Phosphatase (PLAP) Recombinant Rabbit mAb

#71270

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Recombinant Antibody
Knockout Validated
Western blotting analysis using placental alkaline phosphatase (PLAP) antibody (Cat#71270). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with placental alkaline phosphatase (PLAP) antibody (Cat#71270, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using placental alkaline phosphatase (PLAP) antibody (Cat#71270). Placental alkaline phosphatase (PLAP) expression in wild type (WT) and ALPP knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with placental alkaline phosphatase (PLAP) antibody (Cat#71270, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human seminoma using placental alkaline phosphatase (PLAP) antibody (Cat#71270, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with Placental alkaline phosphatase (PLAP) antibody (Cat#71270, 1:1,000). Nuclei were stained blue with DAPI; Placental alkaline phosphatase (PLAP) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Placental alkaline phosphatase (PLAP) expression in HepG2 cells using Placental alkaline phosphatase (PLAP) antibody (Cat#71270, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Placental alkaline phosphatase (PLAP).
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Unit price：
Quantity：
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  • Gene Symbol：
    ALPP
  • Catalog：
    71270
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name KO-Validated Placental Alkaline Phosphatase (PLAP) Recombinant Rabbit mAb
Aliases ALPP; Alkaline Phosphatase, Placental; PLAP; PALP; Alkaline Phosphatase, Placental Type; Alkaline Phosphatase Regan Isozyme; Placental Alkaline Phosphatase 1; EC 3.1.3.1; PLAP-1; Alkaline Phosphatase, Placental (Regan Isozyme); Intestinal-Type Alkaline Phosphatase; Intestinal Alkaline Phosphatase; Placental Alkaline Phosphatase; Alkaline Phosphomonoesterase; Glycerophosphatase; Regan Isozyme; ALPI; ALP; IAP
Background

Gene Name: ALPP

NCBI Gene Entry: 250

UniProt Entry: P05187

Application Information

Molecular Weight: Predicted, 58 kDa; observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4200

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human Placental alkaline phosphatase (PLAP)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using placental alkaline phosphatase (PLAP) antibody (Cat#71270). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with placental alkaline phosphatase (PLAP) antibody (Cat#71270, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using placental alkaline phosphatase (PLAP) antibody (Cat#71270). Placental alkaline phosphatase (PLAP) expression in wild type (WT) and ALPP knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with placental alkaline phosphatase (PLAP) antibody (Cat#71270, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human seminoma using placental alkaline phosphatase (PLAP) antibody (Cat#71270, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HepG2 cells with Placental alkaline phosphatase (PLAP) antibody (Cat#71270, 1:1,000). Nuclei were stained blue with DAPI; Placental alkaline phosphatase (PLAP) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of Placental alkaline phosphatase (PLAP) expression in HepG2 cells using Placental alkaline phosphatase (PLAP) antibody (Cat#71270, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Placental alkaline phosphatase (PLAP).
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ALPP
  • Catalog：
    71270
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using placental alkaline phosphatase (PLAP) antibody (Cat#71270). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with placental alkaline phosphatase (PLAP) antibody (Cat#71270, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using placental alkaline phosphatase (PLAP) antibody (Cat#71270). Placental alkaline phosphatase (PLAP) expression in wild type (WT) and ALPP knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with placental alkaline phosphatase (PLAP) antibody (Cat#71270, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human seminoma using placental alkaline phosphatase (PLAP) antibody (Cat#71270, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with Placental alkaline phosphatase (PLAP) antibody (Cat#71270, 1:1,000). Nuclei were stained blue with DAPI; Placental alkaline phosphatase (PLAP) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Placental alkaline phosphatase (PLAP) expression in HepG2 cells using Placental alkaline phosphatase (PLAP) antibody (Cat#71270, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Placental alkaline phosphatase (PLAP).
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