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KO-Validated DDAH1 Mouse mAb

#71301

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Knockout Validated
Western blotting analysis using DDAH1 antibody (Cat#71301). DDAH1 expression in wild-type (WT) and DDAH1 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DDAH1 antibody (Cat#71301, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol:
    DDAH1
  • Catalog:
    71301
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KO-Validated DDAH1 Mouse mAb
Aliases DDAH1; Dimethylarginine Dimethylaminohydrolase 1; DDAH; N(G),N(G)-Dimethylarginine Dimethylaminohydrolase 1; Dimethylargininase-1; EC 3.5.3.18; DDAH-1; DDAHI; NG, NG-Dimethylarginine Dimethylaminohydrolase; Epididymis Secretory Protein Li 16; HEL-S-16
Background

Gene Name: DDAH1

NCBI Gene Entry: 23576

UniProt Entry: O94760

Application Information

Molecular Weight: Predicted, 31 kDa; observed, 36 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB1700

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human DDAH1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using DDAH1 antibody (Cat#71301). DDAH1 expression in wild-type (WT) and DDAH1 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DDAH1 antibody (Cat#71301, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using DDAH1 antibody (Cat#71301). DDAH1 expression in wild-type (WT) and DDAH1 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DDAH1 antibody (Cat#71301, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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