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Integrin Beta 3/ CD61 Recombinant Rabbit mAb

#7733

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Recombinant Antibody
Western blotting analysis using Integrin β3 / CD61 antibody (Cat#7733). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin β3 / CD61 antibody (Cat#7733, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Integrin beta 3 / CD61 antibody (Cat#7733, 1:1,000). Nuclei were stained blue with DAPI; Integrin beta 3 / CD61 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Integrin beta 3 / CD61 expression in HepG2 cells using Integrin beta 3 / CD61 antibody (Cat#7733, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Integrin beta 3 / CD61.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    ITGB3
  • Catalog:
    7733
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H
Information
Product Name Integrin Beta 3/ CD61 Recombinant Rabbit mAb
Aliases Integrin Subunit Beta 3; GPIIIa; CD61; GP3A; Integrin, Beta 3 (Platelet Glycoprotein IIIa, Antigen CD61); Platelet Membrane Glycoprotein IIIa; Integrin Beta-3; Antigen CD61; Integrin Beta Chain, Beta 3; Platelet Glycoprotein IIIa; Integrin Beta 3; CD61 Antigen; BDPLT16; BDPLT24; BDPLT2; GT2; GT
Background

Gene Name: ITGB3

NCBI Gene Entry: 3690

UniProt Entry: P05106

Application Information

Molecular Weight: Predicted, 87 kDa; observed, 103 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB905

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human Integrin beta 3 / CD61
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Integrin β3 / CD61 antibody (Cat#7733). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin β3 / CD61 antibody (Cat#7733, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Integrin beta 3 / CD61 antibody (Cat#7733, 1:1,000). Nuclei were stained blue with DAPI; Integrin beta 3 / CD61 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Integrin beta 3 / CD61 expression in HepG2 cells using Integrin beta 3 / CD61 antibody (Cat#7733, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Integrin beta 3 / CD61.
Western blotting analysis using Integrin β3 / CD61 antibody (Cat#7733). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin β3 / CD61 antibody (Cat#7733, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Integrin beta 3 / CD61 antibody (Cat#7733, 1:1,000). Nuclei were stained blue with DAPI; Integrin beta 3 / CD61 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Integrin beta 3 / CD61 expression in HepG2 cells using Integrin beta 3 / CD61 antibody (Cat#7733, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Integrin beta 3 / CD61.
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