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Home>Product>Primary Antibody

Sirtuin 6 Recombinant Rabbit mAb

#7761

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Recombinant Antibody
Western blotting analysis using Sirtuin 6 antibody (Cat#7761). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Sirtuin 6 antibody (Cat#7761, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with Sirtuin 6 antibody (Cat#7761, 1:1,000). Nuclei were stained blue with DAPI; Sirtuin 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SIRT6 expression in HT-1080 cells using SIRT6 antibody (Cat#7761, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SIRT6.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SIRT6
  • Catalog：
    7761
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Sirtuin 6 Recombinant Rabbit mAb
Aliases SIRT6; Sirtuin 6; Protein Mono-ADP-Ribosyltransferase Sirtuin-6; NAD-Dependent Protein Deacetylase Sirtuin-6; NAD-Dependent Protein Deacylase Sirtuin-6; Regulatory Protein SIR2 Homolog 6; SIR2-Like Protein 6; SIR2L6; HSIRT6; Sirtuin (Silent Mating Type Information Regulation 2, S. Cerevisiae, Homolog) 6; Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 6 (S. Cerevisiae); Sir2-Related Protein Type 6; Sirtuin Type 6; EC 2.3.1.286; EC 2.3.1.-; EC 2.4.2.-
Background

Gene Name: SIRT6

NCBI Gene Entry: 51548

UniProt Entry: Q8N6T7

Application Information

Molecular Weight: Predicted, 39 kDa; observed, 42 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1135

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human SIRT6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Sirtuin 6 antibody (Cat#7761). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Sirtuin 6 antibody (Cat#7761, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HT-1080 cells with Sirtuin 6 antibody (Cat#7761, 1:1,000). Nuclei were stained blue with DAPI; Sirtuin 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of SIRT6 expression in HT-1080 cells using SIRT6 antibody (Cat#7761, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SIRT6.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SIRT6
  • Catalog：
    7761
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using Sirtuin 6 antibody (Cat#7761). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Sirtuin 6 antibody (Cat#7761, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with Sirtuin 6 antibody (Cat#7761, 1:1,000). Nuclei were stained blue with DAPI; Sirtuin 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SIRT6 expression in HT-1080 cells using SIRT6 antibody (Cat#7761, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SIRT6.
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