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PI3K p110 α Recombinant Rabbit mAb

#6343

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Recombinant Antibody
Western blotting analysis using PI3K p110 α antibody (Cat#6343). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PI3K p110 α antibody (Cat#6343, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PI3 kinase p110 alpha expression in H9c2 cells using PI3 kinase p110 alpha antibody (Cat#6343, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PI3 kinase p110 alpha.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PIK3CA
  • Catalog:
    6343
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name PI3K p110 α Recombinant Rabbit mAb
Aliases PI3K; Phosphatidylinositol 4,5-Bisphosphate 3-Kinase Catalytic Subunit; Alpha Isoform; Phosphoinositide-3-Kinase, Catalytic, Alpha Polypeptide; Serine/Threonine Protein Kinase PIK3CA PtdIns-3-Kinase Subunit P110-Alpha; Phosphoinositide 3-Kinase Alpha; PI3K-Alpha; Phosphatidylinositol-4,5-Bisphosphate 3-Kinase 110 KDa Catalytic Subunit Alpha; Phosphatidylinositol 4,5-Bisphosphate 3-Kinase 110 KDa Catalytic Subunit Alpha; Mutant Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Alpha; Phosphatidylinositol-4,5-Bisphosphate 3-Kinase, Catalytic Subunit Alpha; Phosphatidylinositol 3-Kinase, Catalytic, Alpha Polypeptide; Phosphatidylinositol 3-Kinase, Catalytic, 110-KD, Alpha; Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide; PI3-Kinase P110 Subunit Alpha; PtdIns-3-Kinase Subunit Alpha; PI3-Kinase Subunit Alpha; EC 2.7.1.137; EC 2.7.1.153; EC 2.7.11.1; P110-Alpha; PI3Kalpha; P110alpha; EC 2.7.1 48; CLAPO; CLOVE; MCMTC; CCM4; CWS5; MCAP; MCM
Background

Gene Name: PIK3CA

NCBI Gene Entry: 5290

UniProt Entry: P42336

Application Information

Molecular Weight: Predicted, 124 kDa; observed, 110 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB950

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human PI3K p110 α
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PI3K p110 α antibody (Cat#6343). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PI3K p110 α antibody (Cat#6343, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of PI3 kinase p110 alpha expression in H9c2 cells using PI3 kinase p110 alpha antibody (Cat#6343, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PI3 kinase p110 alpha.
Western blotting analysis using PI3K p110 α antibody (Cat#6343). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PI3K p110 α antibody (Cat#6343, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PI3 kinase p110 alpha expression in H9c2 cells using PI3 kinase p110 alpha antibody (Cat#6343, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PI3 kinase p110 alpha.
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