Western blotting analysis using proliferating cell nuclear antigen antibody (Cat#H1116). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with proliferating cell nuclear antigen antibody (Cat#H1116, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using proliferating cell nuclear antigen antibody (Cat#H1116). Proliferating cell nuclear antigen expression in wild type (WT) and proliferating cell nuclear antigen (PCNA) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with proliferating cell nuclear antigen antibody (Cat#H1116, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using proliferating cell nuclear antigen antibody (Cat#H1116, 1:2,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining shows positive staining in paraffin-embedded human thyroid cancer. proliferating cell nuclear antigen antibody (Cat#H1116) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human bladder cancer. proliferating cell nuclear antigen antibody (Cat#H1116) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human hepatocellular carcinoma. proliferating cell nuclear antigen antibody (Cat#H1116) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human testis. proliferating cell nuclear antigen antibody (Cat#H1116) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human colon. proliferating cell nuclear antigen antibody (Cat#H1116) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Flow cytometric analysis of proliferating cell nuclear antigen expression in Raji cells using proliferating cell nuclear antigen antibody (Cat#H1116, 1:2,000). Green, isotype control; red, proliferating cell nuclear antigen.
Validation of Proliferating cell nuclear antigen knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Proliferating cell nuclear antigen antibody (Cat#H1116, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
