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KD-Validated Cathepsin D LC Rabbit pAb

#64946

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shRNA Knockdown Validated
Western blotting analysis using cathepsin D LC antibody (Cat#64946). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cathepsin D LC antibody (Cat#64946, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using cathepsin D LC antibody (Cat#64946). Cathepsin D LC expression in wild-type (WT) and cathepsin D (CTSD) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with cathepsin D LC antibody (Cat#64946, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    CTSD
  • Catalog:
    64946
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated Cathepsin D LC Rabbit pAb
Aliases CTSD; Cathepsin D; CLN10; CPSD; Ceroid-Lipofuscinosis, Neuronal 10; EC 3.4.23.5; Epididymis Secretory Sperm Binding Protein Li 130P; Cathepsin D (Lysosomal Aspartyl Protease); Lysosomal Aspartyl Peptidase; Lysosomal Aspartyl Protease; HEL-S-130P; EC 3.4.23
Background

Gene Name: CTSD

NCBI Gene Entry: 1509

UniProt Entry: P07339

Application Information

Molecular Weight: Predicted, 45 kDa; observed, 14 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human Cathepsin D LC
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Data
  • Western blotting analysis using cathepsin D LC antibody (Cat#64946). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cathepsin D LC antibody (Cat#64946, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using cathepsin D LC antibody (Cat#64946). Cathepsin D LC expression in wild-type (WT) and cathepsin D (CTSD) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with cathepsin D LC antibody (Cat#64946, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Western blotting analysis using cathepsin D LC antibody (Cat#64946). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cathepsin D LC antibody (Cat#64946, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using cathepsin D LC antibody (Cat#64946). Cathepsin D LC expression in wild-type (WT) and cathepsin D (CTSD) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with cathepsin D LC antibody (Cat#64946, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
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