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Home>Product>Primary Antibody

KO-Validated SOX2 Recombinant Rabbit mAb

#H1124

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Western blotting analysis using SRY-box transcription factor 2 antibody (Cat#H1124). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SRY-box transcription factor 2 antibody (Cat#H1124, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using SRY-box transcription factor 2 antibody (Cat#H1124). SRY-box transcription factor 2 expression in wild-type (WT) and SRY-box transcription factor 2 (SOX2) knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SRY-box transcription factor 2 antibody (Cat#H1124, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining shows negative staining in paraffin-embedded human seminoma. SOX2 antibody (Cat#H1124) was used at 1:2,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. SOX2 antibody (Cat#H1124) was used at 1:2,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human lung. SOX2 antibody (Cat#H1124) was used at 1:2,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human stomach. SOX2 antibody (Cat#H1124) was used at 1:2,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Flow cytometric analysis of SRY-box transcription factor 2 expression in A549 cells using SRY-box transcription factor 2 antibody (Cat#H1124, 1:2,000). Green, isotype control; red, SRY-box transcription factor 2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SOX2
  • Catalog：
    H1124
  • Application：
    WB, IHC-P, FCM
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name KO-Validated SOX2 Recombinant Rabbit mAb
Aliases SOX2; SRY-Box Transcription Factor 2; SRY (Sex Determining Region Y)-Box 2; Transcription Factor SOX-2; SRY-Box 2; Sex Determining Region Y-Box 2; SRY-Related HMG-Box Gene 2; Transcription Factor SOX2; MCOPS3; ANOP3
Background

Gene Name: SOX2

NCBI Gene Entry: 6657

UniProt Entry: P48431

Application Information

Molecular Weight: Predicted, 34 kDa; observed, 35 kDa

Clonality: Recombinant Monoclonal Antibody

Clone ID: SDT-066-58

Species Reactivity: Human ,rat

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human SOX2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunohistochemistry-Paraffin (IHC-P): 1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using SRY-box transcription factor 2 antibody (Cat#H1124). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SRY-box transcription factor 2 antibody (Cat#H1124, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using SRY-box transcription factor 2 antibody (Cat#H1124). SRY-box transcription factor 2 expression in wild-type (WT) and SRY-box transcription factor 2 (SOX2) knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SRY-box transcription factor 2 antibody (Cat#H1124, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining shows negative staining in paraffin-embedded human seminoma. SOX2 antibody (Cat#H1124) was used at 1:2,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. SOX2 antibody (Cat#H1124) was used at 1:2,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows negative staining in paraffin-embedded human lung. SOX2 antibody (Cat#H1124) was used at 1:2,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human stomach. SOX2 antibody (Cat#H1124) was used at 1:2,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Flow cytometric analysis of SRY-box transcription factor 2 expression in A549 cells using SRY-box transcription factor 2 antibody (Cat#H1124, 1:2,000). Green, isotype control; red, SRY-box transcription factor 2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SOX2
  • Catalog：
    H1124
  • Application：
    WB, IHC-P, FCM
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using SRY-box transcription factor 2 antibody (Cat#H1124). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SRY-box transcription factor 2 antibody (Cat#H1124, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using SRY-box transcription factor 2 antibody (Cat#H1124). SRY-box transcription factor 2 expression in wild-type (WT) and SRY-box transcription factor 2 (SOX2) knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SRY-box transcription factor 2 antibody (Cat#H1124, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining shows negative staining in paraffin-embedded human seminoma. SOX2 antibody (Cat#H1124) was used at 1:2,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. SOX2 antibody (Cat#H1124) was used at 1:2,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human lung. SOX2 antibody (Cat#H1124) was used at 1:2,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human stomach. SOX2 antibody (Cat#H1124) was used at 1:2,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Flow cytometric analysis of SRY-box transcription factor 2 expression in A549 cells using SRY-box transcription factor 2 antibody (Cat#H1124, 1:2,000). Green, isotype control; red, SRY-box transcription factor 2.
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