Western blotting analysis using anti-phospho-GSK-3β (Ser9) antibody (Cat#R0006). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-GSK-3β (Ser9) antibody (Cat#R0006, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-phospho-GSK-3β (Ser9) antibody (Cat#R0006). Phospho-GSK-3β (Ser9) expression in wild-type (WT) and GSK3B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-GSK-3β (Ser9) antibody (Cat#R0006, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with anti-Phospho-GSK-3β (Ser9) antibody (Cat#R0006, 1:1,000). Nuclei were stained blue with DAPI; Phospho-GSK-3β (Ser9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-GSK-3β (Ser9) expression in HepG2 cells using anti-Phospho-GSK-3β (Ser9) antibody (Cat#R0006, 1:2,000). Green, isotype control; red, Phospho-GSK-3β (Ser9).
