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Home>Product>Primary Antibody

KD-Validated CREB Rabbit mAb

#R0016

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Western blotting analysis using anti-CREB1 antibody (Cat#R0016). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CREB1 antibody (Cat#R0016, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CREB1 antibody (Cat#R0016). CREB1 expression in wild type (WT) and CREB1 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CREB1 antibody (Cat#R0016, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with anti-CREB1 antibody (Cat#R0016, 1:1,000). Nuclei were stained blue with DAPI; CREB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Flow cytometric analysis of CREB1 expression in C2C12 cells using anti-CREB1 antibody (Cat#R0016, 1:2,000). Green, isotype control; red, CREB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CREB1
  • Catalog：
    R0016
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated CREB Rabbit mAb
Aliases CREB1; CAMP Responsive Element Binding Protein 1; Cyclic AMP-Responsive Element-Binding Protein 1; CREB-1; Cyclic Adenosine 3',5'-Monophosphate Response Element-Binding Protein CREB; Cyclic Adenosine 3',5'-Monophosphate Response Element Binding Protein; CAMP-Responsive Element-Binding Protein 1; CAMP-Response Element-Binding Protein-1; Active Transcription Factor CREB; Transactivator Protein; CREB
Background

Gene Name: CREB1

NCBI Gene Entry: 1385

UniProt Entry: P16220

Application Information

Molecular Weight: Predicted, 35 kDa; observed, 43 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: S-747-60

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human CREB
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using anti-CREB1 antibody (Cat#R0016). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CREB1 antibody (Cat#R0016, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using CREB1 antibody (Cat#R0016). CREB1 expression in wild type (WT) and CREB1 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CREB1 antibody (Cat#R0016, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with anti-CREB1 antibody (Cat#R0016, 1:1,000). Nuclei were stained blue with DAPI; CREB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Flow cytometric analysis of CREB1 expression in C2C12 cells using anti-CREB1 antibody (Cat#R0016, 1:2,000). Green, isotype control; red, CREB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CREB1
  • Catalog：
    R0016
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using anti-CREB1 antibody (Cat#R0016). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CREB1 antibody (Cat#R0016, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CREB1 antibody (Cat#R0016). CREB1 expression in wild type (WT) and CREB1 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CREB1 antibody (Cat#R0016, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with anti-CREB1 antibody (Cat#R0016, 1:1,000). Nuclei were stained blue with DAPI; CREB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Flow cytometric analysis of CREB1 expression in C2C12 cells using anti-CREB1 antibody (Cat#R0016, 1:2,000). Green, isotype control; red, CREB1.
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