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Home>Product>Primary Antibody

DYKDDDDK Tag Recombinant Rabbit mAb

#T025

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Western blotting analysis using flag tag antibody (Cat#T025). Recombinant multi-tag protein (5 ng) in E. coli were loaded and separated by SDS-PAGE. The blot was incubated with flag tag antibody (Cat#T025, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
  • Catalog：
    T025
  • Application：
    WB
  • Species Reactivity：
    Recombinant protein

Information

Data

Information
Product Name DYKDDDDK Tag Recombinant Rabbit mAb
Aliases FLAG tag; DDDDK tag
Background The FLAG tag is a short peptide sequence (DYKDDDDK) commonly used in molecular biology to facilitate the detection and purification of recombinant proteins. Its small size minimizes interference with protein function and can be specifically recognized by anti-FLAG tag antibodies. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
Application Information

Molecular Weight: Recombinant protein dependent

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB7035

Species Reactivity: Recombinant protein

Applications Tested: Western blotting (WB)
Immunogen Recombinant protein of DYKDDDDK-Tag
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.05% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Note This product is for research use only.
Data
  • Western blotting analysis using flag tag antibody (Cat#T025). Recombinant multi-tag protein (5 ng) in E. coli were loaded and separated by SDS-PAGE. The blot was incubated with flag tag antibody (Cat#T025, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
  • Catalog：
    T025
  • Application：
    WB
  • Species Reactivity：
    Recombinant protein
RELATED PRODUCTS
Western blotting analysis using flag tag antibody (Cat#T025). Recombinant multi-tag protein (5 ng) in E. coli were loaded and separated by SDS-PAGE. The blot was incubated with flag tag antibody (Cat#T025, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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