Western blotting analysis using neurofilament medium chain antibody (Cat#4798). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with neurofilament medium chain antibody (Cat#4798, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human skin tissue using neurofilament medium chain antibody (Cat#4798, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of neurofilament medium chain expression in HeLa cells using neurofilament medium chain antibody (Cat#4798, 1:2,000). Green, isotype control; red, Neurofilament medium chain.
Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)
Immunogen
Recombinant protein of human NEFM
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB):1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000 Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note
This product is for research use only.
Data
Western blotting analysis using neurofilament medium chain antibody (Cat#4798). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with neurofilament medium chain antibody (Cat#4798, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human skin tissue using neurofilament medium chain antibody (Cat#4798, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of neurofilament medium chain expression in HeLa cells using neurofilament medium chain antibody (Cat#4798, 1:2,000). Green, isotype control; red, Neurofilament medium chain.
Western blotting analysis using neurofilament medium chain antibody (Cat#4798). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with neurofilament medium chain antibody (Cat#4798, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human skin tissue using neurofilament medium chain antibody (Cat#4798, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of neurofilament medium chain expression in HeLa cells using neurofilament medium chain antibody (Cat#4798, 1:2,000). Green, isotype control; red, Neurofilament medium chain.
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