Western blotting analysis using ACLY antibody (Cat#62146). ACLY expression in wild-type (WT) and ACLY shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ACLY antibody (Cat#62146, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using ACLY antibody (Cat#62146). ACLY expression in wild-type (WT) and ACLY shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ACLY antibody (Cat#62146, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ACLY antibody (Cat#62146). ACLY expression in wild-type (WT) and ACLY shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ACLY antibody (Cat#62146, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000756s ]
SELECT * FROM `goods` WHERE `code` = '62146' LIMIT 1 [ RunTime:0.000457s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000418s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000332s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000385s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 10581 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000316s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000452s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 10581 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000346s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000558s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000364s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.053128s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (9851,6235,4532,10558,4262,4542,7265,5638,196,4027) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000905s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000537s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000239s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000546s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000281s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000366s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000576s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000275s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000263s ]
