Western blotting analysis using IFIT3 antibody (Cat#6257). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IFIT3 antibody (Cat#6257, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HACAT cells with IFIT3 antibody (Cat#6257, 1:1,000) . Nuclei were stained blue with DAPI; IFIT3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of IFIT3 expression in HaCaT cells using IFIT3 antibody (Cat#6257,1:2,000). Green, isotype control; red, IFIT3.