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  • Primary Antibody
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Home>Product>Primary Antibody

KD-Validated NEFL Mouse mAb

#66239

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shRNA Knockdown Validated
Western blotting analysis using neurofilament light chain antibody (Cat#66239). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with neurofilament light chain antibody (Cat#66239, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using neurofilament light chain antibody (Cat#66239). Neurofilament light chain expression in wild-type (WT) and neurofilament light chain (NEFL) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with neurofilament light chain antibody (Cat#66239, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    NEFL
  • Catalog：
    66239
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated NEFL Mouse mAb
Aliases NEFL; Neurofilament Light Chain; NF68; NFL; PPP1R110; CMT1F; CMT2E; Protein Phosphatase 1, Regulatory Subunit 110; Neurofilament, Light Polypeptide 68kDa; Neurofilament Light Polypeptide; Neurofilament Triplet L Protein; NF-L; Light Molecular Weight Neurofilament Protein; Neurofilament Protein, Light Chain; Neurofilament, Light Polypeptide; 68 KDa Neurofilament Protein; Neurofilament Subunit NF-L; Neurofilament Light; CMTDIG
Background

Gene Name: NEFL

NCBI Gene Entry: 4747

UniProt Entry: P07196

Application Information

Molecular Weight: Predicted, 62 kDa; observed, 65 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB8135

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen Recombinant protein of human NEFL
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using neurofilament light chain antibody (Cat#66239). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with neurofilament light chain antibody (Cat#66239, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using neurofilament light chain antibody (Cat#66239). Neurofilament light chain expression in wild-type (WT) and neurofilament light chain (NEFL) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with neurofilament light chain antibody (Cat#66239, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    NEFL
  • Catalog：
    66239
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using neurofilament light chain antibody (Cat#66239). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with neurofilament light chain antibody (Cat#66239, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using neurofilament light chain antibody (Cat#66239). Neurofilament light chain expression in wild-type (WT) and neurofilament light chain (NEFL) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with neurofilament light chain antibody (Cat#66239, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
  154. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_cart.php ( 3.88 KB )
  155. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/foot.php ( 5.05 KB )
  156. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  157. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/response/Html.php ( 0.97 KB )
  158. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.000506s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000795s ]
  3. SELECT * FROM `goods` WHERE `code` = '66239' LIMIT 1 [ RunTime:0.000545s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000554s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000392s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000408s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 9764 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000355s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000479s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 9764 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000344s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000405s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000306s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.053220s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (7266,18,10055,4491,4955,6478,9236,5247,6783,5282) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000827s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000454s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000216s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000586s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000366s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000416s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000444s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000241s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000202s ]
0.096589s